The image shows a developing Drosophila spermatid immunostained for caspase activity (green). TRITC-phalloidin is used to label actin (red). Caspase is required in spermiogenesis for the elimination ...

Shown is a 0.5 micrometer thick confocal image of a midplane section of a 3T3 fibroblast pulsed for 5 min with BrdU and immuno-labeled with anti-BrdU antibodies. Replication is confined to discrete si...

Shown are 0.5 micrometer thick confocal image of midplane sections of synchronized 3T3 fibroblasts pulsed for 2 min with CldU and labeled with FITC (green) followed by a variable chase time, and a sec...

During late anaphase, Tem1 (green) localized to both spindle pole bodies (SPBs), but Bfa1 (red) was asymmetrically localized to the daughter SPB. DAPI (blue) and DIC (hidden) are also shown. Compare t...

Both Tem1 (green) and Bfa1 (red) are localized to both spindle pole bodies in metaphase. DAPI (blue) and DIC (hidden) are also shown. Compare to anaphase image CIL# 13868. Image is Fig 6A, top panels,...

Shown is a chromosome spread of a PtK1 cell pulsed with BrdU in early S phase, then treated with colcemid to arrest cells at metaphase. Spreads were immuno-labeled with FITC-conjugated anti-BrdU antib...

Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows a rebudded cell, CIL #1387...

Tem1 (green) localizes to spindle pole bodies (SPB) during metaphase. Control image for CIL# 13876 in which Tem1 loading onto SPBs was severely affected in bfa1∆ cells. DAPI (blue) and DIC (hidden) ...

Tem1-CAAX (green) is targeted away from spindle pole bodies in wild-type cells (this image) but can also still localize to the SPB (CIL# 13881). The CAAX motif from Ras2 targets proteins to the cytopl...

Tem1 (green) loading onto spindle pole bodies during anaphase is severely affected in bfa1Δ cells. Compare with control image CIL# 13871. DAPI (blue) and DIC (hidden) are also shown. Image is Fig 6B,...